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Protein Purification by HisTrip Column and 6XHis Tag TEV Cleavage Frozen bacterial pellets were resuspended in the lysis buffer (50 mM Tris-HCl, 150 mM NaCl, pH 8.0), and the cells were lysed using an ultrasonic processor

Missouri Med 115(3):219 Niimi M, Murao K (2005) Neuropeptide W as a stress mediator in the hypothalamus

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Omega-3 polyunsaturated fatty acids and inflammatory processes: Nutrition or pharmacology

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