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On the other hand, GSH does not react directly non-enzymatically with hydroperoxides

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Therefore, GSSG in the sample solution can be selectively determined by measuring the absorption (max = 412 nm) derived from a colorimetric reaction of DTNB (5,5-dithiobis (2-nitrobenzoic acid)) coupled with the enzymatic recycling system

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We've found that chronic studies often require careful monitoring for any potential compensatory mechanisms