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RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

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Recognizing the unique pain pattern and affected areas is crucial for accurate diagnosis and timely treatment of Trigeminal Neuralgia, especially as early intervention can help prevent worsening symptoms and improve quality of life