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In contrast, persistently stabilized NRF2 in response to KEAP1 or NRF2 mutations causes sustained activation of these genes, leading to the accumulation of xCT/SLC7A11, GCLC, and GCLM that cause a depletion of intracellular glutamate (Sayin et al

Based on prior experience with this kit, to ensure proper mixing of reagents, we placed the 96-well plate on an orbital plate shaker during incubation steps

The results showed that, compared with those in response to either natural product alone, most DEGs in both pathways were synergistically regulated by the combination (Fig

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