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The total glutathione (GSH) levels were based on the reaction of GSH with 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) (Ellmans reagent), which forms an oxidized glutathione (GSSG)2-nitro-5-thiobenzoic acid (TNB) product that is later reduced by glutathione reductase in the presence of NADPH with the consequent synthesis of GSH

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