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The 2.0 mL dilution gives a clean 1.0 mg/mL concentration where 1 unit equals 10 mcg, so every dose reads on a round U-100 mark

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Positive control mice each received PADRE-J8 (30 g) emulsified in CFA and PBS (1:1, total 50 L) for the primary immunisation followed by three boosts of PADRE-J8 (30 g) in PBS (50 L)

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However, individual experiences vary, and some people do develop skin reactions that warrant medical attention