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Subsequently, 250-m-thick slices of the hindbrain containing the AP were cut on a Vibratome (Leica) and immersed in a recovery solution (in mM: 3 KCl, 118 NaCl, 25 NaHCO 3 , 1.2 NaH 2 PO 4 , 2.5 Glucose, 7 MgCl 2 , 0.5 CaCl 2

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The findings by Saenger et al[38] that systemic delivery of IGF-I was beneficial for mice with a milder but not severe phenotype of ALS suggest that IGF-I may have some limited neuron protective effect, but not sufficient to cure the disease

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It helps regulate hunger and fullness so you eat less without feeling deprived