doi: 10.1016/j.nutres.2010.04.004 7
Sections were fixed with 4% paraformaldehyde, rinsed with PBS, blocked with goat serum (Vector laboratories, CA, USA) for 30 min at room temperature, and incubated with mouse-anti-GSK-3/ monoclonal antibody (SC-7291, 1:50, Santa Cruz, TX, USA) at 4 C overnight
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