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Reproductive abnormalities were not observed in GPx3 knockout mice [18], but reduced cell proliferation and increased rates of cell death were found in GPx3 knockout Xenopus laevis embryos [19]

Real-time polymerase chain reaction (PCR) was performed by using a kit (SYBR Premix EX Taq, Takara Bio Inc.) and the ABI PRISM 7300 real-time PCR system