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In recent years, the establishment of cross-tissue single-cell atlases has systematically revealed the lineage and functional heterogeneity of human monocytes/macrophages in health and disease, providing an important reference framework for analyzing metabolism-phenotype coupling at the human level (135)

Yes, different facial areas and body regions often benefit from different concentrations

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The lysate was subsequently centrifuged (12000 g, 4 C, 5 min), denatured, analysed by SDSPAGE, and transferred to PVDF membranes (IPVH15150, Millipore)