For TOF MS analysis, scan time for the MS e method (centroid) was set to 0.1 sec, high-resolution negative ionization mode and the following ion source parameters were selected: capillary voltage (-2.5 kV), sampling cone (50 V), source temperature (150 C), desolvation temperature (450 C), cone gas (10 L/h) and desolvation gas (850 L/h)
Assay: GSH/GSSG in human HepG2 cells
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GPx1but not GSS or GSRknockdown (Supplementary Fig