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Variants such as rAAV-PHP.B and a second generation rAAV-PHP.eB contain an engineered capsidcontributing to an unprecedented efficiency in crossing the BBB upon intravenous injection, along with high diffusion capacity into both neurons and glia of mice [163,164,165,166]

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In some embodiments, a composition or formulation disclosed herein has an ionic balance that is the same as or substantially the same as the endolymph