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To collect details on subcellular localisation of the GFP signals reporting Nt TPC1A, we conducted spinning disc confocal microscopy using an AxioObserver Z1 microscope equipped with a laser dual spinning disk scan head from Yokogawa (Yokogawa CSU X1 Spinning Disk Unit, Yokogawa Electric Corporation, Tokyo, Japan), and a cooled digital CCD camera (AxioCam MRm
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If the condition is severe, a person can even break a bone by sneezing, coughing or making small movements
Serum cotinine levels, smoking and periodontal attachment loss
