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Mechanistically, IRE1s endoribonuclease activity cleaves and down-regulates the mRNA of key glutathione biosynthesis regulators glutamate-cysteine ligase catalytic subunit (GCLC) and solute carrier family 7 member 11 (SLC7A11)

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This feature was exploited for selective and sensitive quantification of creatinine (CRN) which is an important diagnostic parameter