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Waist circumference and waist-to-hip ratio provide supplementary tracking metrics

In humans, CCK dose-dependently presents early satiety and reduces the energy intake at a buffet style meal, which was attenuated by the CCK-1 antagonist, loxiglumide (107)

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sgRNAs against exons 4 and 5 of the Gipr , Glp-1r and Gcgr genes (ESM Table 2) were designed using CRISPOR tool (www.crispor.tefor.net), in vitro transcribed (E3322, NEB) and purified using the MEGAclear Kit

The cuproptosis-related gene PDHA1 may serve as a node linking these pathways, regulating lipid metabolism through its interaction with the PI3K-Akt-mTOR signaling pathway [123] (Figure 5)

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