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After incubation, 1 mL of absolute EtOH was added and SP3 beads were magnetically captured and washed three times with 80% EtOH, then resuspended in 200 L 50 mM HEPES buffer (pH 7.4) containing 1 mM TCEP and incubated for 3 min at RT

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10.1186/1471-2180-10-140 BMC Microbiol

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